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Saturday, May 2, 2020 | History

4 edition of A novel cell culture model to study ischemia-reperfusion injury in lung transplantation found in the catalog.

A novel cell culture model to study ischemia-reperfusion injury in lung transplantation

Jonathan A. Cardella

A novel cell culture model to study ischemia-reperfusion injury in lung transplantation

by Jonathan A. Cardella

  • 236 Want to read
  • 19 Currently reading

Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 1999.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL20329178M
ISBN 100612461068
OCLC/WorldCa47644302

  Based on novel insights in cold-induced cell and tissue injury [7,8], we have recently developed an improved new modification of the former Custodiol solution, which proved to be of superior quality in cell culture experiments as well as preservation of liver and lung Cited by:   1 Introduction. Lung transplantation is an established therapy of end-stage lung disease. However, the outcome of this complex procedure is still impaired by relatively high complication rates mostly due to ischemia/reperfusion (I/R) injury Cited by:

  The majority of renal ischemic/reperfusion (I/R) and ischemic postconditioning (IPO) studies have been based on animal models. To gain mechanistic insights into ischemic postconditioning-induced alterations at the cell level, a novel in vitro model of I/R and IPO is set up by using the rat proximal tubule cell line NRKE. Cells Cited by: 4.   In the present study we therefore used a rat single-lung transplant model to examine apoptotic and necrotic cell death after IR injury. With this model we were able to subject the donor lung Cited by:

Rationale: Ischemia–reperfusion (IR) injury after lung transplantation, which affects both short- and long-term allograft survival, involves activation of NADPH oxidase 2 (NOX2) and activation of invariant natural killer T (iNKT) cells to produce IL Adenosine A 2A receptor (A 2AR) agonists are known to potently attenuate lung IR injury. IRI, ischemia reperfusion injury; LPC, lung progenitor cell; LTx, lung transplantation; NOX, NADPH oxidase PS, pneumosphere; ROS, reactive oxygen species Received 09 September , revised 09 March and accepted for publication 11 March Introduction Chronic lung .


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A novel cell culture model to study ischemia-reperfusion injury in lung transplantation by Jonathan A. Cardella Download PDF EPUB FB2

A novel cell culture model for studying ischemia-reperfusion injury in lung transplantation. J Appl Physiol –, —Many cell culture models have been developed to study ischemia. Lung transplantation has emerged as a therapeutic moddity for patients with end-stage lung disease. However ischemia reperfiision injury remains a significant clinical problem, occurring in 20% of patients.

Many ce11 culture models have been developed to study ischemia-reperfüsion (VR) injury Cited by: Liver cubes in culture are a model for the hepatic lobule, which uncouples ischemia from reperfusion through differences in the distribution of injury.

This novel technique will assist in understanding the underlying molecular mechanisms that both separate and unite : Bernard J. DuBray, Kendra D. Conzen, Gundumi A. Upadhya, Parvathi Balachandran, Jianluo Jia, Brett L. In the present study, we designed a novel double-layer parallel-plate flow chamber (PPFC) to develop an in vitro ischemia/reperfusion (I/R) injury model and examined the effects of I/R on inflammatory responses in human microvascular endothelial cells Cited by: ischemia and ex vivo perfusion in lung transplantation.

A novel cell culture model for study-ing ischemia–reperfusion injury in lung transplantation. J Appl. Novel Approaches to Preventing Ischemia-Reperfusion Injury During Liver Transplantation Article Literature Review in Transplantation Proceedings 45(6) July with Reads.

Although anoxia/reoxygenation of cultured cells has been used to model lung ischemia–reperfusion injury, this does not accurately mimic events experienced by lung cells while a lung is retrieved from Cited by: 9.

While this intervention limits ischaemic injury, it triggers a new cascade of events that is also harmful, viz. reperfusion injury. In recent years, novel insights have emerged regarding mechanisms of ischaemia–reperfusion injury Cited by: Pulmonary ischemia–reperfusion injury (IRI) causes postoperative morbidity in patients undergoing lung transplantation, isolated lung perfusion, and cardiopulmonary bypass and may lead to potentially lethal pathologies such as respiratory shock.

In-depth study of this pathology requires a reliable animal by: 4. Conclusions: A homodimer of recombinant annexin V reduced ischemia reperfusion injury in a lung transplant animal model, by reducing cell death and tissue inflammation.

Although anoxia/reoxygenation of cultured cells has been employed to model lung ischemia-reperfusion injury (IRI), this does not accurately mimic events experienced by lung cells while a lung is retrieved from a donor, stored, and transplanted.

We developed an in vitro model of non-hypoxic IRI Cited by: 9. Autologous mitochondrial transplantation provides a novel technique to significantly enhance myocardial cell viability following ischemia and reperfusion in the clinically relevant swine by: Stem cell-derived mitochondria transplantation: a novel strategy and the challenges for the treatment of tissue injury Jingyu Wang1, Heyangzi Li2, Ying Yao1, Tengfei Zhao3, Ying-ying Chen2, Yue-liang Shen2, Lin-lin Wang2* and Yongjian Zhu1* Abstract Damage of mitochondria in the initial period of tissue injury aggravates the severity of by: 6.

Hypoxia–reoxygenation of cultured macrovascular endothelial cells is used to study ischemia–reperfusion (IR)-related cellular and molecular changes; however, these models do not accurately depict events in pulmonary microvascular endothelial cells (PMVECs) during conventional lung retrieval Cited by: Renal ischemia reperfusion injury is a pathological event that can lead to acute renal failure (ARF), and found that 45% of the cases of ARF in the intensive care unit due to IRI in one study.

Mesenchymal stem cells (MSCs) suppress inflammation and immune responses. We conducted this study to find out if MSCs attenuate ischemia–reperfusion injury in a mouse model of lung transplantation. C57BL/6J mouse lungs perfused with low-potassium dextran glucose solution were preserved at 4 °C for 18 h.

Human MSCs were slowly injected into the left pulmonary artery of the lung Cited by: 7. We designed an experimental protocol of ischemia/reperfusion in order to allow the observation of cellular connections and even subcellular mechanisms during ischemia/reperfusion injury and after stem cell transplantation and to evaluate the efficacy of cell therapy.

H9c2 cardiomyoblast cells were placed onto cell culture Cited by: 3. Though uptake by alveolar macrophages has been demonstrated, studies have not examined its use in lung ischemia reperfusion injury.

We describe the validation of short interference RNA as a novel Cited by: 3. Purpose In lung transplantation, ischemia-reperfusion injury (IRI) plays a critical role in the development of primary graft dysfunction.

Among cell deaths induced by IRI, regulated necrosis has. Ischemic tolerance has been described in a variety of primary cell culture system, but similar in vitro model for renal ischemia reperfusion injury have not been well established.

The present study. Apoptosis induced by reactive oxygen species is the initial step in IRI. We therefore investigated whether the IRI-reducing effect of MSC-Exo or miRp as described in Fig. 1, Fig. 2 was mediated by reducing apoptosis in the I/R model lung. Western blot results showed that intratracheal treatment of MSC-Exo significantly reduced Caspase-3 activation in I/R model Cited by: RNA as a novel technique for cell-specific target gene knockdown in our model of lung ischemia–reperfusion injury.

Methods: Dose-response experiments were performed, and 3 distinct se- .Background: Lung ischemia-reperfusion (IR) injury after transplantation as well as acute shortage of suitable donor lungs are two critical issues impacting lung transplant patients.

This study investigates the anti-inflammatory and immunomodulatory role of human mesenchymal stromal cells .